The RRRC genotypes all lines upon arrival. When provided, genotyping protocols supplied by donating investigators are validated and optimized. When no genotyping protocol exists, the RRRC develops new gene-specific assays using a variety of techniques including standard PCR, RFLP analysis, High-Resolution Melt (HRM), RT-PCR, qPCR and Taqman (probe-based) methods.
For lines involving unique or uncharacterized genetic backgrounds and/or lines developed for QTL analysis which do not involve discrete genetic mutations, protocols involving genome-wide microsatellite analysis are developed.
Genotyping protocols for individual rat models are available under "genetic description" in the strain profile.
RRRC genotyping protocols have been optimized for the reagents and conditions described in the protocols. To genotype animals, the RRRC recommends researchers validate the assays independently upon receipt of animals into their facility. Reaction cycling temperatures and times may require additional optimization based on the specific genotyping reagents and instrumentation used.
Please contact us if you have any questions or need assistance- our genotyping experts are alway available for consultation and can help you trouble-shoot your genotyping assays.
05.21.2026
Weather and Shipping
The welfare of the animals is of utmost importance and as we head into the summer months, inclement weather and excessive heat will delay/prevent shipments of live animals. Be aware that hot temperatures between June and mid-September may negatively impact the ability of couriers to safely ship animals so please plan your live animal orders accordingly.
05.21.2026
Provide Your Input on NIH's Proposed Strategic Plan by May 26, 2026.
The NIH is currently seeking input for its next strategic plan, and a major focus of this framework involves the future of animal research. Because these high-level policies directly impact the future of the RRRC and your work, it’s crucial that the scientific community participates. The NIH has provided a short feedback form that only takes a few minutes to complete. Please consider submitting your input. URL: https://grants.nih.gov/
07.30.2025
Animal Models and New Approach Methodologies (NAMs)
NAMs are lab (e.g., organoids and iPSCs) or computer-based methods intended to expand our tools for modeling human biology and disease. While this field is rapidly evolving, the need for animal models remains, especially for situations where a complex biological system is necessary. In addition, animal models serve as vital in vivo controls for the validation and verification of emerging NAMs.