The RRRC is one of the few groups with expertise to isolate and culture rat embryonic stem cells (ESCs). In addition, we are continually investigating ways to improve rat ESC culture conditions. We are available for consultation for investigators who want to use rat ESCs in their research and we can isolate new rat ESC lines and/or generate genetically modified rat ESCs for making rat models on a fee-for-service basis.
RRRC Rat embryonic stem cell culture and cryopreservation protocol
Relevant RRRC Publications
Effect of Gsk3 inhibitor CHIR99021 on aneuploidy levels in rat embryonic stem cells. Bock AS, Leigh ND, Bryda EC. (2014). In Vitro Cell Dev Biol Anim.50:572-579.
Derivation of a Germline Competent Transgenic Fischer 344 Embryonic Stem Cell Line. Men, H and Bryda, EC. (2013) PLoS One 8:e56518.
Germline Transmisson of a Novel Rat Embryonic Stem Cell Line Derived from Transgenic rats. Men H. Bauer BA, Bryda EC. (2012) Stem Cells Dev. 21(14):2606-2612.
05.21.2026
Weather and Shipping
The welfare of the animals is of utmost importance and as we head into the summer months, inclement weather and excessive heat will delay/prevent shipments of live animals. Be aware that hot temperatures between June and mid-September may negatively impact the ability of couriers to safely ship animals so please plan your live animal orders accordingly.
05.21.2026
Provide Your Input on NIH's Proposed Strategic Plan by May 26, 2026.
The NIH is currently seeking input for its next strategic plan, and a major focus of this framework involves the future of animal research. Because these high-level policies directly impact the future of the RRRC and your work, it’s crucial that the scientific community participates. The NIH has provided a short feedback form that only takes a few minutes to complete. Please consider submitting your input. URL: https://grants.nih.gov/
07.30.2025
Animal Models and New Approach Methodologies (NAMs)
NAMs are lab (e.g., organoids and iPSCs) or computer-based methods intended to expand our tools for modeling human biology and disease. While this field is rapidly evolving, the need for animal models remains, especially for situations where a complex biological system is necessary. In addition, animal models serve as vital in vivo controls for the validation and verification of emerging NAMs.