for assistance call: 1-888-673-3444
 
Microbiota Characterization Now Offered
 July 07, 2014
The RRRC now offers a full line of NGS-related services including fecal DNA extraction, 16S rDNA gene sequencing, and informatics analysis to researchers using rat models.  For more information, please see here or contact www.rrrc.us.
Need a New Rat Model?
 March 21, 2017
Need a new rat model?  The RRRC in conjunction with the MU Animal Modeling Core (AMC) can assist with the generation of transgenic rats, create knock-out and knock-in rats using CRISPR/Cas9 technology and genetically manipulate rat embryonic stem cells to make chimeric animals.  Contact us to discuss your rat model needs and how we can help.
Genotyping
The RRRC genotypes all lines upon arrival.  When provided, genotyping protocols supplied by donating investigators are validated and optimized.  When no genotyping protocol exists, the RRRC develops new gene-specific assays using a variety of techniques including standard PCR, RFLP analysis, RT-PCR, qPCR and Taqman (probe-based) methods.

For lines involving unique or uncharacterized genetic backgrounds and/or lines developed for QTL analysis which do not involve discrete genetic mutations, protocols involving genome-wide microsatellite analysis are developed.  

Genotyping protocols for individual rat models are available under "genetic description" in the strain profile.